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Assessment of the anti-inflammatory capacity of CPS gel in vitro . A Schematic of the co-culture of hydrogel with RAW 264.7. B Quantification of the relative fluorescence intensity of TNF-α. C Quantification of the relative fluorescence intensity of <t>IL-1β.</t> D Immunofluorescence image of TNF-α expression in RAW 264.7 (Scale bar = 50 μm). E Immunofluorescence image of IL-1β expression in RAW 264.7 (Scale bar = 50 μm). F-H The protein expression level of TNF-α and IL-1β was evaluated by WB and quantified by ImageJ. Data are mean ± SD (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Il 1β, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Assessment of the anti-inflammatory capacity of CPS gel in vitro . A Schematic of the co-culture of hydrogel with RAW 264.7. B Quantification of the relative fluorescence intensity of TNF-α. C Quantification of the relative fluorescence intensity of <t>IL-1β.</t> D Immunofluorescence image of TNF-α expression in RAW 264.7 (Scale bar = 50 μm). E Immunofluorescence image of IL-1β expression in RAW 264.7 (Scale bar = 50 μm). F-H The protein expression level of TNF-α and IL-1β was evaluated by WB and quantified by ImageJ. Data are mean ± SD (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Anti Il 1β, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Assessment of the anti-inflammatory capacity of CPS gel in vitro . A Schematic of the co-culture of hydrogel with RAW 264.7. B Quantification of the relative fluorescence intensity of TNF-α. C Quantification of the relative fluorescence intensity of <t>IL-1β.</t> D Immunofluorescence image of TNF-α expression in RAW 264.7 (Scale bar = 50 μm). E Immunofluorescence image of IL-1β expression in RAW 264.7 (Scale bar = 50 μm). F-H The protein expression level of TNF-α and IL-1β was evaluated by WB and quantified by ImageJ. Data are mean ± SD (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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Assessment of the anti-inflammatory capacity of CPS gel in vitro . A Schematic of the co-culture of hydrogel with RAW 264.7. B Quantification of the relative fluorescence intensity of TNF-α. C Quantification of the relative fluorescence intensity of <t>IL-1β.</t> D Immunofluorescence image of TNF-α expression in RAW 264.7 (Scale bar = 50 μm). E Immunofluorescence image of IL-1β expression in RAW 264.7 (Scale bar = 50 μm). F-H The protein expression level of TNF-α and IL-1β was evaluated by WB and quantified by ImageJ. Data are mean ± SD (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Antibodies Against Il 1β, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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Assessment of the anti-inflammatory capacity of CPS gel in vitro . A Schematic of the co-culture of hydrogel with RAW 264.7. B Quantification of the relative fluorescence intensity of TNF-α. C Quantification of the relative fluorescence intensity of IL-1β. D Immunofluorescence image of TNF-α expression in RAW 264.7 (Scale bar = 50 μm). E Immunofluorescence image of IL-1β expression in RAW 264.7 (Scale bar = 50 μm). F-H The protein expression level of TNF-α and IL-1β was evaluated by WB and quantified by ImageJ. Data are mean ± SD (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Journal: Materials Today Bio

Article Title: Ultrasound-responsive CPS piezoelectric hydrogel synergistically repairs annulus fibrosus defects through immune reprogramming and cell recruitment

doi: 10.1016/j.mtbio.2026.102825

Figure Lengend Snippet: Assessment of the anti-inflammatory capacity of CPS gel in vitro . A Schematic of the co-culture of hydrogel with RAW 264.7. B Quantification of the relative fluorescence intensity of TNF-α. C Quantification of the relative fluorescence intensity of IL-1β. D Immunofluorescence image of TNF-α expression in RAW 264.7 (Scale bar = 50 μm). E Immunofluorescence image of IL-1β expression in RAW 264.7 (Scale bar = 50 μm). F-H The protein expression level of TNF-α and IL-1β was evaluated by WB and quantified by ImageJ. Data are mean ± SD (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Staining was performed using IL-1β (Bioss, BS-0812R) and TNF-α (Santa, SC-52746) probes, and the staining was observed under a fluorescent inverted microscope.

Techniques: In Vitro, Co-Culture Assay, Fluorescence, Immunofluorescence, Expressing

In vivo study of CPS gel for AF repair. A H&E, S&O staining sections of rat IVD at 4W and 8W post-operation (scale bar = 1 mm). B Col-1, IL-1β IHC staining sections of rat IVD at 4W and 8W post-operation (Scale bar = 50 μm). C Postoperative 4W Col-1 quantitative analysis. D Postoperative 8W Col-1 quantitative analysis. E. Postoperative 4W IL-1β quantitative analysis. F Postoperative 8W IL-1β quantitative analysis. Data are mean ± SD (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Journal: Materials Today Bio

Article Title: Ultrasound-responsive CPS piezoelectric hydrogel synergistically repairs annulus fibrosus defects through immune reprogramming and cell recruitment

doi: 10.1016/j.mtbio.2026.102825

Figure Lengend Snippet: In vivo study of CPS gel for AF repair. A H&E, S&O staining sections of rat IVD at 4W and 8W post-operation (scale bar = 1 mm). B Col-1, IL-1β IHC staining sections of rat IVD at 4W and 8W post-operation (Scale bar = 50 μm). C Postoperative 4W Col-1 quantitative analysis. D Postoperative 8W Col-1 quantitative analysis. E. Postoperative 4W IL-1β quantitative analysis. F Postoperative 8W IL-1β quantitative analysis. Data are mean ± SD (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Staining was performed using IL-1β (Bioss, BS-0812R) and TNF-α (Santa, SC-52746) probes, and the staining was observed under a fluorescent inverted microscope.

Techniques: In Vivo, Staining, Immunohistochemistry